Saline:Article Title: Sustained release delivery systems comprising traceless linkers
Article Snippet: .. Cells were lysed in 1× Tris-buffered saline (50 mM Tris-HCl, pH 7.5, 150 mM NaCl), 1% Triton X-100, 5 mM EDTA, 1× protease phosphatase inhibitor (Thermoscientific) for 30-45 min on a horizontal shaker at 4° C. The samples were stored overnight at −80° C. IGF-1R phosphorylation levels were analyzed by ELISA using the DuoSet IC human phosphor-IGF-1R (R&D systems) with the following modifications: the capture antibody (R&D MAB391) was diluted to 4 ug/ml in PBS, wells were washed with 300 ul/well of PBS-Tween (Millipore, 524653), wells were blocked with 200 ul/well 1% BSA in PBS, phosphorylation was detected using an antibody HRP-anti-phosphotyrosine (R&D, HAM1676) and the luminescent signal was detected with a chemiluminescence substrate (Pierce, 37069). .. Cells were lysed in 1× Tris-buffered saline (50 mM Tris-HCl, pH 7.5, 150 mM NaCl), 1% Triton X-100, 5 mM EDTA, 1× protease phosphatase inhibitor (Thermoscientific) for 30-45 min on a horizontal shaker at 4° C. The samples were stored overnight at −80° C. IGF-1R phosphorylation levels were analyzed by ELISA using the DuoSet IC human phosphor-IGF-1R (R&D systems) with the following modifications: the capture antibody (R&D MAB391) was diluted to 4 ug/ml in PBS, wells were washed with 300 ul/well of PBS-Tween (Millipore, 524653), wells were blocked with 200 ul/well 1% BSA in PBS, phosphorylation was detected using an antibody HRP-anti-phosphotyrosine (R&D, HAM1676) and the luminescent signal was detected with a chemiluminescence substrate (Pierce, 37069).
Article Title: Sustained release delivery systems comprising traceless linkers
Article Snippet: .. Cells were lysed in 1×Tris-buffered saline (50 mM Tris HCl, pH 7.5, 150 mM NaCl), 1% Triton X-100, 5 mM EDTA, 1×protease phosphatase inhibitor (Thermoscientific) for 30-45 min on a horizontal shaker at 4° C. The samples were stored overnight at −80° C. IGF-1R phosphorylation levels were analyzed by ELISA using the DuoSet IC human phosphor-IGF-1R (R&D systems) with the following modifications: the capture antibody (R&D MAB391) was diluted to 4 ug/ml in PBS, wells were washed with 300 ul/well of PBS-Tween (Millipore, 524653), wells were blocked with 200 ul/well 1% BSA in PBS, phosphorylation was detected using an antibody HRP-anti-phosphotyrosine (R&D, HAM1676) and the luminescent signal was detected with a chemiluminescence substrate (Pierce, 37069). ..
Phospho-proteomics:Article Title: Sustained release delivery systems comprising traceless linkers
Article Snippet: .. Cells were lysed in 1× Tris-buffered saline (50 mM Tris-HCl, pH 7.5, 150 mM NaCl), 1% Triton X-100, 5 mM EDTA, 1× protease phosphatase inhibitor (Thermoscientific) for 30-45 min on a horizontal shaker at 4° C. The samples were stored overnight at −80° C. IGF-1R phosphorylation levels were analyzed by ELISA using the DuoSet IC human phosphor-IGF-1R (R&D systems) with the following modifications: the capture antibody (R&D MAB391) was diluted to 4 ug/ml in PBS, wells were washed with 300 ul/well of PBS-Tween (Millipore, 524653), wells were blocked with 200 ul/well 1% BSA in PBS, phosphorylation was detected using an antibody HRP-anti-phosphotyrosine (R&D, HAM1676) and the luminescent signal was detected with a chemiluminescence substrate (Pierce, 37069). .. Cells were lysed in 1× Tris-buffered saline (50 mM Tris-HCl, pH 7.5, 150 mM NaCl), 1% Triton X-100, 5 mM EDTA, 1× protease phosphatase inhibitor (Thermoscientific) for 30-45 min on a horizontal shaker at 4° C. The samples were stored overnight at −80° C. IGF-1R phosphorylation levels were analyzed by ELISA using the DuoSet IC human phosphor-IGF-1R (R&D systems) with the following modifications: the capture antibody (R&D MAB391) was diluted to 4 ug/ml in PBS, wells were washed with 300 ul/well of PBS-Tween (Millipore, 524653), wells were blocked with 200 ul/well 1% BSA in PBS, phosphorylation was detected using an antibody HRP-anti-phosphotyrosine (R&D, HAM1676) and the luminescent signal was detected with a chemiluminescence substrate (Pierce, 37069).
Article Title: Tough Composite Hydrogels with High Loading and Local Release of Biological Drugs
Article Snippet: Finally, cells were lysed in 1× TBS, 1× TritonX-100, 0.005M EDTA, 1× protease phosphatase inhibitor (Thermo Scientific) for 30–45 minutes on a horizontal shaker at +4°C. .. IGF-1R phosphorylation was analyzed by ELISA using the same procedure as the DuoSet IC human phosphor-IGF-1R (R&D systems) with the following modifications: the capture antibody (R&D MAB391) was diluted to 4 ug/ml in PBS, wells were washed with 300ul/well of PBS-Tween (Millipore, 524653), wells were blocked with 200ul/well 1% BSA in PBS, phosphorylation was detected using an antibody HRP-anti-phosphotyrosine (R&D, HAM1676) and the luminescent signal was detected with a chemiluminescence substrate (Pierce, 37069). .. Adult female Sprague Dawley rats (age: 9 months; body weight: 300 to 400 g; supplier: Janvier) were housed according to standard conditions with food and water ad libitum and light/dark cycle of 12/12 hours.
Article Title: Sustained release delivery systems comprising traceless linkers
Article Snippet: .. Cells were lysed in 1×Tris-buffered saline (50 mM Tris HCl, pH 7.5, 150 mM NaCl), 1% Triton X-100, 5 mM EDTA, 1×protease phosphatase inhibitor (Thermoscientific) for 30-45 min on a horizontal shaker at 4° C. The samples were stored overnight at −80° C. IGF-1R phosphorylation levels were analyzed by ELISA using the DuoSet IC human phosphor-IGF-1R (R&D systems) with the following modifications: the capture antibody (R&D MAB391) was diluted to 4 ug/ml in PBS, wells were washed with 300 ul/well of PBS-Tween (Millipore, 524653), wells were blocked with 200 ul/well 1% BSA in PBS, phosphorylation was detected using an antibody HRP-anti-phosphotyrosine (R&D, HAM1676) and the luminescent signal was detected with a chemiluminescence substrate (Pierce, 37069). ..
Enzyme-linked Immunosorbent Assay:Article Title: Sustained release delivery systems comprising traceless linkers
Article Snippet: .. Cells were lysed in 1× Tris-buffered saline (50 mM Tris-HCl, pH 7.5, 150 mM NaCl), 1% Triton X-100, 5 mM EDTA, 1× protease phosphatase inhibitor (Thermoscientific) for 30-45 min on a horizontal shaker at 4° C. The samples were stored overnight at −80° C. IGF-1R phosphorylation levels were analyzed by ELISA using the DuoSet IC human phosphor-IGF-1R (R&D systems) with the following modifications: the capture antibody (R&D MAB391) was diluted to 4 ug/ml in PBS, wells were washed with 300 ul/well of PBS-Tween (Millipore, 524653), wells were blocked with 200 ul/well 1% BSA in PBS, phosphorylation was detected using an antibody HRP-anti-phosphotyrosine (R&D, HAM1676) and the luminescent signal was detected with a chemiluminescence substrate (Pierce, 37069). .. Cells were lysed in 1× Tris-buffered saline (50 mM Tris-HCl, pH 7.5, 150 mM NaCl), 1% Triton X-100, 5 mM EDTA, 1× protease phosphatase inhibitor (Thermoscientific) for 30-45 min on a horizontal shaker at 4° C. The samples were stored overnight at −80° C. IGF-1R phosphorylation levels were analyzed by ELISA using the DuoSet IC human phosphor-IGF-1R (R&D systems) with the following modifications: the capture antibody (R&D MAB391) was diluted to 4 ug/ml in PBS, wells were washed with 300 ul/well of PBS-Tween (Millipore, 524653), wells were blocked with 200 ul/well 1% BSA in PBS, phosphorylation was detected using an antibody HRP-anti-phosphotyrosine (R&D, HAM1676) and the luminescent signal was detected with a chemiluminescence substrate (Pierce, 37069).
Article Title: Tough Composite Hydrogels with High Loading and Local Release of Biological Drugs
Article Snippet: Finally, cells were lysed in 1× TBS, 1× TritonX-100, 0.005M EDTA, 1× protease phosphatase inhibitor (Thermo Scientific) for 30–45 minutes on a horizontal shaker at +4°C. .. IGF-1R phosphorylation was analyzed by ELISA using the same procedure as the DuoSet IC human phosphor-IGF-1R (R&D systems) with the following modifications: the capture antibody (R&D MAB391) was diluted to 4 ug/ml in PBS, wells were washed with 300ul/well of PBS-Tween (Millipore, 524653), wells were blocked with 200ul/well 1% BSA in PBS, phosphorylation was detected using an antibody HRP-anti-phosphotyrosine (R&D, HAM1676) and the luminescent signal was detected with a chemiluminescence substrate (Pierce, 37069). .. Adult female Sprague Dawley rats (age: 9 months; body weight: 300 to 400 g; supplier: Janvier) were housed according to standard conditions with food and water ad libitum and light/dark cycle of 12/12 hours.
Article Title: Sustained release delivery systems comprising traceless linkers
Article Snippet: .. Cells were lysed in 1×Tris-buffered saline (50 mM Tris HCl, pH 7.5, 150 mM NaCl), 1% Triton X-100, 5 mM EDTA, 1×protease phosphatase inhibitor (Thermoscientific) for 30-45 min on a horizontal shaker at 4° C. The samples were stored overnight at −80° C. IGF-1R phosphorylation levels were analyzed by ELISA using the DuoSet IC human phosphor-IGF-1R (R&D systems) with the following modifications: the capture antibody (R&D MAB391) was diluted to 4 ug/ml in PBS, wells were washed with 300 ul/well of PBS-Tween (Millipore, 524653), wells were blocked with 200 ul/well 1% BSA in PBS, phosphorylation was detected using an antibody HRP-anti-phosphotyrosine (R&D, HAM1676) and the luminescent signal was detected with a chemiluminescence substrate (Pierce, 37069). ..
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